Our DNA authentication assay distinguishes between natural and artificial DNA samples. The assay receives as input a DNA sample that is processed biochemically followed by automatic signal analysis, and outputs a decision whether the sample is natural or artificial. An internal validation step also controls for amplification failure due to PCR inhibitors, insufficient template DNA, etc. The first generation assay is based on detection, quantification, and sodium bisulfite sequencing of specific target genomic loci, and analysis of their methylation patterns. The second generation assay, currently under development, employs different biochemical and analysis tools achieving a more comprehensive solution. The assay is designed as a kit that can be integrated into the forensic procedure, requiring minimal training of personnel and standard equipment. As part of the development process, the kit is routinely tested on natural DNA samples and on different types of artificial DNA samples, synthesized by a variety of in-vitro methods.